Estimated Reading Time: 15 minutes This review explores the effect of scaffold technology and nanomaterials on antibody biosensors’ performance. These innovations increase the sensitivity of biosensors for rapidly identifying bacterial pathogens in healthcare and environmental safety. The article discusses the combination of new materials and designs in creating these biosensors, their challenges, and their potential. It compares traditional and advanced biosensors, highlights the use of nanotechnology, and looks at different detection methods, such as electrochemical and optical techniques, to recognize bacteria. Furthermore, this review emphasizes the importance of developing future antibody biosensors for diagnostics and treatment.